Automated whole-cell patch clamp electrophysiology of neurons in vivo
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Kodandaramaiah, S., Talei Franzesi, G., Chow, B., Boyden, E. S.*, Forest, C.* (2012) Automated whole-cell patch clamp electrophysiology of neurons in vivo, Nature Methods 9:585–587. (* co-corresponding authors)
Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis
of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform.
We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting
cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield,
throughput and quality of automated intracellular recording in mouse cortex and hippocampus.
Resources associated with this Publication:
[Autopatching: In Vivo Robotics, for Automatic Recording of Neurons in the Live Brain]