Arch: Archaerhodopsin from H. sodomense: genetically-encoded reagent for high-performance yellow/green-light neural silencing
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FCK-Arch-GFP Plasmid for: lentiviral excitatory neuron expression of Arch-GFP. Contains: 2nd-generation lentiviral backbone, CaMKII promoter, yellow/green-light driven proton pump Arch (Archaerhodopsin from H. sodomense, aka ar-3), GFP. |
[Source publication] [VectorNTI file] [Detailed map] [Gene and plasmid sequence] [Virus making protocol] |
[Request at Addgene] [You can also email Prof. Boyden to request, to ask for virus, or to see if updates are available] |
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AAV-FLEX-Arch-GFP Plasmid for: adeno-associated virus (AAV) Cre-dependent cell-type selective expression of Arch-GFP. Contains: AAV-FLEX backbone (Atasoy et al., J. Neurosci., 28:7025-7030, 2008, used with permission), CAG promoter, reversed cassette with yellow/green-light driven proton pump Arch (Archaerhodopsin from H. sodomense, aka ar-3) and GFP. |
[Source publication] [VectorNTI file] [Detailed map] [Gene and plasmid sequence] |
[Request at Addgene] [You can also email Prof. Boyden to request, to ask for virus, or to see if updates are available] |
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FCK-ss-Prl-Arch-GFP Plasmid for: lentiviral excitatory neuron expression of ss-Prl-Arch-GFP. Contains: CaMKII promoter, ss-Prl enhanced trafficking sequence, yellow/green-light driven proton pump Arch (Archaerhodopsin from H. sodomense, aka ar-3), GFP. Slightly higher currents than Arch. |
[Source publication] [VectorNTI file] [Detailed map] [Gene and plasmid sequence] [Virus making protocol] |
[Request at Addgene] [You can also email Prof. Boyden to request, to ask for virus, or to see if updates are available] |
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FCK-ss-Arch-GFP-ER2 Plasmid for: lentiviral excitatory neuron expression of ss-Arch-GFP-ER2. Contains: CaMKII promoter, ss enhanced trafficking sequence, yellow/green-light driven proton pump Arch (Archaerhodopsin from H. sodomense, aka ar-3), GFP, ER2 enhanced trafficking sequence. Currents are of the same magnitude as Arch. |
[Source publication] [VectorNTI file] [Detailed map] [Gene and plasmid sequence] [Virus making protocol] |
[Email Prof. Boyden to request, to ask for virus, or to see if updates are available] |
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FCK-Arch-CFP Plasmid for: lentiviral excitatory neuron expression of Arch-CFP. Contains: 2nd-generation lentiviral backbone, CaMKII promoter, yellow/green-light driven proton pump Arch (Archaerhodopsin from H. sodomense, aka ar-3), CFP. |
[Source publication] [VectorNTI file] [Detailed map] [Gene and plasmid sequence] [Virus making protocol] |
[Request at Addgene] [You can also email Prof. Boyden to request, to ask for virus, or to see if updates are available] |