Synthetic Neurobiology Group

Ed Boyden, Ph.D., Principal Investigator

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Protocols and Reagents

Optical Neural Control ('Optogenetics'): Reagents

  • Arch: Archaerhodopsin from H. sodomense: genetically-encoded reagent for high-performance yellow/green-light neural silencing

  • Mac: opsin from L. maculans: genetically-encoded reagent for blue/green-light neural silencing

  • ChR2-2A-Halo: Channelrhodopsin-halorhodopsin gene fusions: bi-directional neural control with two different colors of light

  • Halo: Halorhodopsin from N. pharaonis: genetically-encoded reagent for yellow-light neural hyperpolarization

  • ChR2: Channelrhodopsin-2 from C. reinhardtii: genetically-encoded reagent for blue-light neural activation

Optical Neural Control ('Optogenetics'): Hardware

  • Very Simple Off-The-Shelf Laser and Viral Injector Systems for In Vivo Optical Neuromodulation

  • Optical Fiber/Laser System for In Vivo (Multicolor) Light Delivery for Brain Neuromodulation

Molecular Biology Protocols

  • Lentivirus production for high-titer, cell-specific, in vivo neural labeling

Home » Protocols and Reagents » Molecular Biology Protocols

Molecular Biology Protocols

Lentivirus production for high-titer, cell-specific, in vivo neural labeling

Copyright 1995-2010, Ed Boyden esb@media.mit.edu